superfrost plus microslides  (Avantor)

Journal: GeroScience

Article Title: Neurobehavioral dysfunction in a mouse model of Down syndrome: upregulation of cystathionine β-synthase, H 2 S overproduction, altered protein persulfidation, synaptic dysfunction, endoplasmic reticulum stress, and autophagy

doi: 10.1007/s11357-024-01146-8

Figure Lengend Snippet: Reactive astrogliosis in the DS brain. Immunohistochemical staining of mouse brain sections of wild-type and DS mice (with or without AOAA treatment) was analyzed in the CA1 region of the hippocampus. Images were acquired using confocal microscopy in 3D at × 40 magnification and are shown as maximum intensity projections of the whole z-stack of 30 μm. Immunofluorescence labeling for marker of astrocytes GFAP (green), CBS (red), and counterstaining with DAPI (blue) is shown. A Representative images; B astrocyte density; C Sholl analysis of astrocyte intersections. Each bar represents the mean ± SEM of 10 animals per group; ** p ≤ 0.01. DS was associated with reactive astrogliosis, as evidenced by higher astrocyte numbers, larger astrocyte cell size, and more complex branching

Article Snippet: Sections were counterstained with 1 μg/ml DAPI (Merck, MBD0015) for the last 5 min. After the final wash, sections were transferred to microscopy slides (MicroSlides SuperFrost® Plus Menzel Epredia, ThermoScientific, Huberlab, 10.0344.01) and mounted with a drop of ProLongTM Gold Antifade Mountant (ThermoFisher Scientific, P36930).

Techniques: Immunohistochemical staining, Staining, Confocal Microscopy, Immunofluorescence, Labeling, Marker

Journal: GeroScience

Article Title: Neurobehavioral dysfunction in a mouse model of Down syndrome: upregulation of cystathionine β-synthase, H 2 S overproduction, altered protein persulfidation, synaptic dysfunction, endoplasmic reticulum stress, and autophagy

doi: 10.1007/s11357-024-01146-8

Figure Lengend Snippet: CBS expression is primarily localized in astrocytes in the mouse hippocampus. Immunohistochemical staining of wild-type mouse brain sections was analyzed in 4 different regions of the hippocampus (CA1, sr-slm, DG, CA3). Images in the top row were acquired using NanoZoomer fully automated slide scanner in 2D at × 5 magnification. Images in the other rows were acquired using confocal microscopy in 3D at × 40 magnifications and are shown as maximum intensity projections of the whole z-stack of 30 μm. Immunofluorescence labeling for marker of astrocytes GFAP (green), CBS (red), and counterstaining with DAPI (blue) is shown. Note the colocalization of CBS with GFAP in all regions of the hippocampus

Article Snippet: Sections were counterstained with 1 μg/ml DAPI (Merck, MBD0015) for the last 5 min. After the final wash, sections were transferred to microscopy slides (MicroSlides SuperFrost® Plus Menzel Epredia, ThermoScientific, Huberlab, 10.0344.01) and mounted with a drop of ProLongTM Gold Antifade Mountant (ThermoFisher Scientific, P36930).

Techniques: Expressing, Immunohistochemical staining, Staining, Confocal Microscopy, Immunofluorescence, Labeling, Marker